https://pages.htgaa.org/2026a/sara-gaviria-escobar/projects/individual-final-project/fp-documentation/index.htmlFINAL PRESENTATION SLIDES SECTION 1: ABSTRACT MycoBoard addresses the global e-waste crisis by engineering the fungi Neurospora crassa to grow biodegradable hyphal mats that function as breadboard-like electronic substrates. Conventional FR4 fiberglass PCBs contribute to the 62 million metric tons of annual e-waste, most of which is non-recyclable. MycoBoard leverages fungal thigmotropism to form conductive tracks within molded mats, with cmt metallothionein overexpression driving copper ion capture along the hyphae walls of N.crassa. The purpose is to create compostable electronic substrates that can break down in soil within weeks. The principle, validated by a Benchling-designed linear cassette and literature-based copper-loading estimates, states that engineered N. crassa could both biosorb copper and form conductive pathways via filamentous formation of hyphae. Aim 1 designs and validates a linear cassette construct for copper-responsive expression of cmt. Aim 2 transforms N. crassa experimentally, grows mats in breadboard molds, and tests LED circuit conductivity. Aim 3 replaces molds with optogenetic cmt control for pattern-directed copper deposition. Methods include Benchling DNA design, Twist synthesis, spheroplast electroporation, fungal mat cultivation, and multimeter/resistance validation.Hugoen