Homework
Weekly homework submissions:
Week 1 HW: Engineering a Soil Microbe to Support Short-Season Agriculture in Canada
Biological engineering application: PGPR Agriculture in northern climates, such as Canada, faces structural biological constraints, particularly short and unpredictable growing seasons, cold soil temperatures in early spring, and variable nutrient availability. These constraints limit crop maturation time and increase vulnerability to climate change. I propose developing an engineered plant growth-promoting rhizobacterium (PGPR) to enhance early-stage plant growth, nutrient uptake, and cold-stress resilience. The literature has recognized the effectiveness of PGPR in improving the growth and quality of certain crops and plants (Singh et al, 2023; Zhang et al, 2024). However, the theories have not been applied to improving the early developmental rate in cold climates. Therefore, I believe this biological engineering approach not only leverages rhizosphere ecology but also aligns agricultural productivity and ecological systems thinking.
Week 2 HW: DNA Read, White, and Edit
Part 1: Benchling & In-silico Gel Art Enzymes Cuts Temp. 1.1 2.1 3.1 4/CS BamHI 5 37°C 75* 100* 100 100* EcoRI 5 37°C 25 100* 50 50* EcoRV 21 37°C 10 50 100 10 HindIII 7 37°C 25 100 50 50 KpnI 2 — Not available for this vendor SacI 2 — Not available for this vendor SacII 4 37°C 10 100 10 100 I have difficulty creating a pattern/image in the style of Paul Vanouse’s Latent Figure Protocol artworks. I will use this image as a placeholder for now. Sorry about that.
from opentrons import types metadata = { # see https://docs.opentrons.com/v2/tutorial.html#tutorial-metadata ‘author’: ‘Scarlett Kelly’, ‘protocolName’: ‘Art’, ‘description’: ‘Making art with Opentrons’, ‘source’: ‘HTGAA 2026 Opentrons Lab’, ‘apiLevel’: ‘2.20’ } ############################################################################## Robot deck setup constants - don’t change these ############################################################################## TIP_RACK_DECK_SLOT = 9 COLORS_DECK_SLOT = 6 AGAR_DECK_SLOT = 5 PIPETTE_STARTING_TIP_WELL = ‘A1’ well_colors = { ‘A1’ : ‘Red’, ‘B1’ : ‘Green’, ‘C1’ : ‘Orange’ }