<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 11 HW: Bioproduction &amp; Cloud Labs :: 2026a-sean-murphy</title><link>https://pages.htgaa.org/2026a/sean-murphy/homework/week-11-hw-bioproduction-and-cloud-labs/index.html</link><description>Cell-Free Protein Synthesis Lab — Questions &amp; Answers Q1. Provide a 1–2 sentence description of each component’s role in the 20-hour NMP-Ribose-Glucose master mix. E. coli Lysate BL21 (DE3) Star Lysate — Provides the core transcription/translation machinery (ribosomes, tRNAs, aminoacyl-tRNA synthetases, initiation/elongation/release factors, and metabolic enzymes). The “Star” strain carries an RNase E mutation that stabilizes mRNA, and the (DE3) lysogen supplies T7 RNA Polymerase for high-level transcription from T7 promoters.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/sean-murphy/homework/week-11-hw-bioproduction-and-cloud-labs/index.xml" rel="self" type="application/rss+xml"/></channel></rss>