Labs
Lab writeups:
I was sick this week.
Week 2 Lab: Gel Electrophoresis
Gel Electrophoresis Designs Our group tried to make a design with the letters “HA” which stands for the name of one of our group members, Hines Alayah. Instead, we somehow ended up with “LU”. Sometimes discoveries in biology are sometimes made serendipitously, so we have decided that “LU” means Love U. Here are a few photo highlights below.
OpenTron Designs I tried to push OpenTron to the limit and chose a fairly hard design. Specifically, I chose the Mitsudomoe design, which is a type of “Kamon” or traditional family crest, associated with my family. The design didn’t come out particularly well but with higher resolution and/or non-sequential pipetting (for speed) it would be a more tractable design.
Please refer to the homework this week.
Week 5 Lab: Protein Design Part 2
Please refer to the homework this week.
Week 6 Lab: Genetic Circuits Part 1
Gibson Assembly Lab This week we performed a Gibson Assembly to clone chromophore-mutant inserts into the mUAV backbone. Here are some photo highlights from the lab. Setting up the PCR reactions — pipetting primers, template, and master mix into tubes Loading samples into the E-Gel EX Invitrogen cassette for gel electrophoresis
Week 7 Lab: Genetic Circuits Part 2
This week we designed a 2-layer intracellular neural network circuit and simulated its behavior. Our team designed a comet. The heatmap of the circuit’s predicted output across X1 and X2 input space produced a comet-shaped gradient, with high expression concentrated in the low-X1/low-X2 corner and a tail fading diagonally across the landscape. Circuit design spreadsheet: our poly-transfection mix with Csy4, CasE, mNeonGreen, and fluorescent markers
Week 09 Lab: cell free systems
Week 09 lab for cell free systems.
Lab Day at Waters Immerse Schematic of the Waters LC-MS instrument setup, our roadmap for the day’s experiments The team suited up in lab coats and safety goggles at the Waters facility Benchside doodle, someone’s artistic interpretation of the day’s science between runs
Week 11 Lab: Bioproduction & Cloud Labs
Week 11 lab for bioproduction & cloud labs.