Week 6 Lab: Genetic Circuits Part 1

Gibson Assembly Lab

This week we performed a Gibson Assembly to clone chromophore-mutant inserts into the mUAV backbone. A few photo highlights from the lab.

Setting up the PCR reactions: pipetting primers, template, and master mix into tubes. PCR reaction setup PCR reaction setup

Loading samples into the E-Gel EX Invitrogen cassette for gel electrophoresis. Loading the E-Gel cassette Loading the E-Gel cassette

Miniprep station: spinning down cultures to extract plasmid DNA. Miniprep station Miniprep station

Gel results: checking PCR product sizes on the 1% agarose E-Gel. PCR gel results PCR gel results

Our gel after DpnI digestion and cleanup. Bands are visible in lanes 1 and 4. Gel after DpnI digestion Gel after DpnI digestion