Week 6 Lab: Genetic Circuits Part 1

Gibson Assembly Lab

This week we performed a Gibson Assembly to clone chromophore-mutant inserts into the mUAV backbone. Here are some photo highlights from the lab.

Setting up the PCR reactions — pipetting primers, template, and master mix into tubes

Loading samples into the E-Gel EX Invitrogen cassette for gel electrophoresis

Miniprep station — spinning down cultures to extract plasmid DNA

Gel results — checking PCR product sizes on the 1% agarose E-Gel

Our gel after DpnI digestion and cleanup — bands visible in lanes 1 and 4