Week 7 Lab: Genetic Circuits Part 2

This week we designed a 2-layer intracellular neural network circuit and simulated its behavior. Our team designed a comet. The heatmap of the circuit’s predicted output across X1 and X2 input space produced a comet-shaped gradient, with high expression concentrated in the low-X1/low-X2 corner and a tail fading diagonally across the landscape.

Circuit design spreadsheet: our poly-transfection mix with Csy4, CasE, mNeonGreen, and fluorescent markers Circuit design spreadsheet Circuit design spreadsheet

Simulation output: the “comet” heatmap showing predicted mNeonGreen expression across X1 and X2 input doses Comet heatmap simulation Comet heatmap simulation

Opentrons deck loaded with tube racks and tip boxes for automated transfection mix preparation Opentrons setup Opentrons setup