https://pages.htgaa.org/2026a/sean-murphy/labs/week-07-lab-genetic-circuits-part-2/index.htmlThis week we designed a 2-layer intracellular neural network circuit and simulated its behavior. Our team designed a comet. The heatmap of the circuit’s predicted output across X1 and X2 input space produced a comet-shaped gradient, with high expression concentrated in the low-X1/low-X2 corner and a tail fading diagonally across the landscape. Circuit design spreadsheet: our poly-transfection mix with Csy4, CasE, mNeonGreen, and fluorescent markersHugoen