https://pages.htgaa.org/2026a/selin-erdem/homework/week-06-hw-genetic-circuit-part-i/index.htmlAssignment: DNA Assembly Answer these questions about the protocol in this week’s lab: What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The Phusion Master Mix is a convenient 2X concentrated solution designed for high-performance PCR. Key components include: Phusion DNA Polymerase: A high-fidelity enzyme with a processivity-enhancing domain, ensuring extremely low error rates and fast extension times. dNTPs: The essential building blocks (dATP, dCTP, dGTP, dTTP) required for new DNA strand synthesis. Reaction Buffer: Maintains the optimal pH and provides necessary ions.$ MgCl2: Acts as a vital cofactor for the polymerase enzyme activity. What are some factors that determine primer annealing temperature during PCR? The Ta is critical for primer specificity and yield. It is primarily determined by:Hugoen