https://pages.htgaa.org/2026a/sergio-cuiza/labs/lab-week-9--cell-free-systems/index.htmlCompletion status: This lab was completed theoretically (no physical or virtual wet lab performed). All procedures, results, and analyses below are based on the provided protocol and scientific literature. The homework questions are answered in full. Overview & Objective In this lab, we demonstrate the functionality of a Cell-Free Transcription-Translation (TXTL) system using an E. coli extract. We express the reporter protein amilGFP from a T7-IPTG‑inducible plasmid. IPTG acts as an inducer by inhibiting the LacI repressor, allowing T7 RNA polymerase to transcribe the gene. The goal is to quantify amilGFP production at different IPTG concentrations over an 8‑hour incubation at 30°C, using fluorescence measurement (ex 492 nm / em 506 nm) either in a plate reader or via end‑point imaging.Hugoen