https://pages.htgaa.org/2026a/shitong-luo/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion DNA Polymerase: synthesizes new DNA strands by adding nucleotides to the template strand during PCR dNTPs (deoxynucleotides): building blocks of DNA Buffer: provides the optimal conditions 2. What are some factors that determine primer annealing temperature during PCR? Tm (melting temperature) of the primer: the temperature at which half of the DNA duplex dissociates Primer length: longer primers generally require higher annealing temperatures GC content: higher GC content increases the Tm and may require higher annealing temperatures Salt concentration: higher salt concentrations can stabilize the DNA duplex and may require higher annealing temperatures 3. There are two methods from this class that create linear fragments of DNA: PCR, and restriction enzyme digests. Compare and contrast these two methods, both in terms of protocol as well as when one may be preferable to use over the other. PCR:Hugoen