https://pages.htgaa.org/2026a/shrimay-saikia/homework/week-09-hw-cell-free-systems/index.htmlHomework Part A Assignment 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell free expression is more beneficial than cell production. It lacks the stress response, proteostasis and detoxification of an in vivo environment, allowing for much higher expression of protein including toxic varieties or those incorporating xenobiotic compounds, as in bio-orthogonal experiments. Reaction conditions are standard and can be externally controlled/varied (eg temperature) more precisely than in in vivo systems. The progress of reaction is more easily followed using imaging techniques, allowing for rapid optimization and sequential syntheses. Two cases where cell-free expression is better than cell production are the expression of protein toxic to bacterial/fungal chassis (eg antibacterial/antifungal compounds) as well as proteins intended for use in human medicine (free of endotoxins).Hugoen