Week 11 HW: Building Genomes

Part A

I enjoyed the sense of oneness and how it spoke to the power of science to transcend divisions of identity and geography

Part B: Cell-Free Protein Synthesis | Cell-Free Reagents

E. coli Lysate: Provides biological medium and machinery (eg ribosomes) Salts/Buffer: Optimal pH, ionic atmosphere and osmolarity Energy / Nucleotide System: Power transcription/translation Translation Mix (Amino Acids): Allow for translation to produce mRNA from DNA Additives: Nicotinamide in particular allows for the regeneration of NAD Backfill: Maintain appropriate volume for manipulation of the biological systems

Part C: Planning the Global Experiment | Cell-Free Master Mix Design

sfGFP: Maturation time - 13.6min mRFP1: Maturation time - 60.0min mKO2: Maturation time - 108.0min mTurquoise2: Maturation time - 33.5min mScarlet_I: Maturation time - 174.0min Electra2: Lifetime - 3.4ns

Increasing the concentrations of AMP, UMP, CMP and GMP across all the proteins will enhance the total fluorescence over the incubation period by increasing the rate of reaction at the rate-limiting-step of translation.