Individual Final Project

1. As part of your final project, design one or more strategies to ensure that your project, and what it enables, contributes to growing an ethical biological future.

2. Fully-annotated plasmid

3. Project rationale, design considerations, biosafety considerations, and engineering goal

4. Iteration

5. TLDR

• pH reporter (external)
• pH modifier?

For your final project:

Please identify at least one (ideally many) aspect(s) of your project that you will measure. It could be the mass or sequence of a protein, the presence, absence, or quantity of a biomarker, etc. Please describe all of the elements you would like to measure, and furthermore describe how you will perform these measurements. What are the technologies you will use (e.g., gel electrophoresis, DNA sequencing, mass spectrometry, etc.)? Describe in detail

REPORTER

• https://www.fpbase.org/protein/rrvt/

As an expansive substitute to traditional geochemical sampling, and cheap complement to existing agritech workflows, SoilBuddy is synbio’s answer to the need for real-time soil health monitoring in humanity’s quest to end world hunger. SoilBuddy captures key metrics such as oxygenation, pH and macronutrient levels through an authentically biological chassis, and conveys actionable insights through naturally-occurring reporter systems. SoilBuddy scales naturally over the space of a farm while implementing safety switches which prevent ecological leakage and facilitate regulatory compliance.

The first aim of my final project is to engineer an AND genetic circuit which produces chemiluminescence in response to high cell density and pH stress.