<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: hw-genetic-circuits-part-i :: 2026a-siwei-zhang</title><link>https://pages.htgaa.org/2026a/siwei-zhang/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Week 6 — Genetic Circuits I: DNA Assembly Technologies
Molecular Biology Lab Report: PCR &amp; Assembly Techniques
Components of Phusion High-Fidelity PCR Master MixPhusion Master Mix is a convenient 2X concentrated solution containing:Phusion DNA Polymerase: A pyrococcus-like enzyme fused with a processivity-enhancing domain. It provides extremely high fidelity ($50\times$ higher than Taq) and speed.dNTPs: The building blocks ($dATP, dTTP, dCTP, dGTP$) for the new DNA strand.Reaction Buffer: Maintains optimal pH and provides ionic strength.MgCl2: A necessary cofactor for polymerase activity.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/siwei-zhang/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>