Projects

Final projects:

  • Memorandum of Understanding (MoU) HTGAA Committed Listener (CL) Agreement This Memorandum of Understanding (MoU) defines the mutual commitment, expectations, and responsibilities between the HTGAA Committed Listener (CL), the Local Node / MOM Lab, and the HTGAA Course Administration. By signing this agreement, the CL acknowledges that HTGAA (How to Grow Almost Anything) is an intensive, graduate-level course requiring strict adherence to laboratory safety, academic integrity, and rigorous resource management.
  • L-Protein Engineering | Option 1: Mutagenesis ☀️ Team Members LIAO LITING WANG YUXIN ZHANG SIWEI Important Objective “Engineering the MS2 Lysis Protein to enhance mutagenesis efficiency while balancing cellular viability—a significant challenge in modern synthetic biology.” 1. Chaperone-independent lysis design; 2. Rapid and efficient E. coli killing; 3. Potentiated lysis protein yield; Fig 1. Electron micrograph of bacteriophages showing their characteristic morphology.
  • HTGAA 2026: Individual Final Project Documentation 🤴The Prometheus Symbiont🤴 SECTION 1: ABSTRACT Provide a concise, self-contained summary of your project (minimum 150 words) The abstract should allow a reader to understand the purpose, approach, and expected outcomes of the work without referring to other sections. Abstract The Prometheus Symbiont is initially proposed as an ideal system based on the principles of natural photosynthesis and a continuous directed evolution platform. Aimed at mimicking natural systems, the ideal concept involves converting photosynthetic membranes into bio-self-powered mechanical systems, thereby enabling robots to replenish their own energy by simulating the foraging behavior of the leaf sheep (Costasiella kuroshimae).
Final Group Project Module →

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Technical Roadmap

1. DnaJ-Independent Mutagenesis

  • Site Identification: Targeting 4 residues in the lysis protein.
  • Engineering: Mutating to remove DnaJ dependency.
  • Validation: Verifying activity across diverse environments.

2. De Novo Protein Design

  • Optimization: Designing for a “mild yet potent” profile.
  • Precision Control:
    • Intensity: Enhancing lytic strength for efficacy.
    • Timing: Calibrating thresholds for accurate release.
  • Clinical Safety: Balancing clearance and host-cell impact.

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Final Individual Project Module →

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HTGAA 2026: Individual Final Project Documentation

SECTION 1: ABSTRACT

SECTION 2: PROJECT AIMS

SECTION 3: BACKGROUND

SECTION 4: EXPERIMENTAL DESIGN, TECHNIQUES, TOOLS, AND TECHNOLOGY

SECTION 5: Results & Quantitative Expectations

SECTION 6: ADDITIONAL INFORMATION

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