https://pages.htgaa.org/2026a/sofya-rakitina/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Based on Protocol for Phusion™ High-Fidelity PCR Master Mix with GC Buffer (https://www.neb.com/en-gb/protocols/protocol-for-phusion-high-fidelity-pcr-master-mix-with-gc-buffer-m0532) Phusion DNA Polymerase - a high-fidelity enzyme that synthesises new DNA strands with 3’→5’ exonuclease activity for proofreading dNTPs - building blocks for DNA synthesis (200 µM each at 1X concentration) MgCl₂ - Cofactor required for polymerase activity (1.5 mM at 1X concentration) Possibly optional as the question doesn’t mention it (GC Buffer - optimised buffer for amplifying difficult templates with high GC content or secondary structure) DMSO (optional additive) - helps denature secondary structures in GC-rich or difficult templates (recommended at 3% final concentration)Hugoen