https://pages.htgaa.org/2026a/sophia-irina-harcau/homework/week-06-genetic_circuits/index.htmlWhat are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The components in the Phusion High-Fidelity PCR Master Mix are Phusion DNA Polymerase, deoxynucleodites, reaction buffer. -Phusion DNA Polymerase-copies the DNA -Reaction buffer-maintains optimal biochemical proprieties What are some factors that determine primer annealing temperature during PCR? -specific annealing enzyme use and their optimal catalysing activity temperature; There are two methods from this class that create linear fragments of DNA: PCR, and restriction enzyme digests. Compare and contrast these two methods, both in terms of protocol as well as when one may be preferable to use over the other.Hugoen