https://pages.htgaa.org/2026a/srinidhi-vasan/homework/week-06-genetic-circuits-part-i/index.htmlDNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Standard Phusion 2X Master Mix contains the following essential components: Phusion High-Fidelity DNA Polymerase: A specialized, proofreading enzyme coupled to a processivity-enhancing domain. Its purpose is to catalyze DNA synthesis with high speed and extremely low error rates. dNTPs (dATP, dCTP, dGTP, dTTP): The nucleotide building blocks required by the polymerase to synthesize the new complementary DNA strands. Phusion HF Buffer: An optimized reaction buffer containing MgCl2. Magnesium (Mg2+) acts as an important cofactor for the DNA polymerase enzyme, stabilizing the reaction and facilitating the smooth incorporation of dNTPs. 2. What are some factors that determine primer annealing temperature during PCR?Hugoen