https://pages.htgaa.org/2026a/sydney-francis/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly (Question 1) Within Phusion High-Fidelity Master Mix [1], there is a Phusion DNA Polymerase (which enzymatically synthesizes the DNA in the 5’ to 3’ direction), nucleotides (the building blocks of the synthesized DNA), and an optimized reaction buffer (maintains optimal conditions for the polymerase). (Question 2) Some factors that determine primer annealing temperature are the primer length, the GC content, and the salt concentration.Hugoen