https://pages.htgaa.org/2026a/sydney-francis/homework/week-10-advanced-imaging-and-measurement-technology/index.htmlFinal Project For my final project, I will need to sequence the genetic circuit that I ultimately construct as well as the concentration of the IL-10, Decorin, and Bxb1 and PhiC31 integrase that is produced by the circuit. In order to sequence the genertic circuit, the most common method would be to use Sanger Sequencing, which utilizes electrophoresis after the synthesis in order to properly sort and sequence the circuit based on lengths and the base that terminated sequencing In order to measure the concentrations of the IL-10, Decorin, and Bxb1 and PhiC31 integrase produced by my genetic circuit, I can use Mass Spectroscopy. After harvesting the expression cells at the appropriate time points, I will use the spike-in standards strategy and then calculate the ratio of my endogenous peptide signal to the heavy standard signal, calculating the concentration based on the moles of the protein measured divided by the volume of my original sample. Waters Pt. 1: Molecular Weight For the following calculations, I will be using the provided eGFP sequenceHugoen