https://pages.htgaa.org/2026a/taliana-vyaltseva/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High‑Fidelity DNA Polymerase. This is the key enzyme that copies DNA during PCR. Reaction Buffer (HF Buffer). The buffer provides the chemical environment in which the enzyme works best. Deoxynucleoside Triphosphates (dNTPs). These are the building blocks of DNA (A, T, G, C). Optional Additives Water. Used to bring the reaction to the final volume and ensure proper concentrations of all components once primers and DNA template are added. 2. What are some factors that determine primer annealing temperature during PCR?Hugoen