https://pages.htgaa.org/2026a/taliana-vyaltseva/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Direct control of conditions: You can independently adjust pH, temperature, ion concentration, redox state, and cofactors without affecting cell viability. Faster optimization cycles: No need for cloning, transformation, or cell growth—results can be obtained in hours. No cellular constraints: Toxic proteins, unstable proteins, or proteins that burden cells can still be produced. Simplified system: Fewer regulatory pathways means fewer unknown biological interactions. Cases where cell-free is more beneficial:Hugoen