https://pages.htgaa.org/2026a/tammy-sisodiya/homework/week-05-hw-protein-design-part-ii/index.htmlPart 1: Generate Binders with PepMLM Changed 5th character from A to V. >sp|P00441|SOD1_HUMAN_A4V Superoxide dismutase [Cu-Zn] OS=Homo sapiens OX=9606 GN=SOD1 PE=1 SV=2 MATK**V**VCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTS AGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVV HEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ 2. Sequence 5 TKGNAGSRLACG WRGDDSVDFEGR 18.929941 Part 2: Evaluate Binders with AlphaFold3 Record the ipTM score and briefly describe where the peptide appears to bind. Does it localize near the N-terminus where A4V sits? Does it engage the β-barrel region or approach the dimer interface? Does it appear surface-bound or partially buried? Protein-peptide sequence 1: SSTLRLFAQLRR, ipTM = 0.55, pTM = 0.67 The low ipTM of 0.3 suggests the peptide is surface-bound, and is roughly associated with the outer beta barrel part. It is not buried and is situated on the exterior surface of the Alphafold model, and does not situate near the N-terminus where the A4V mutation sits.Hugoen