https://pages.htgaa.org/2026a/tammy-sisodiya/homework/week-06-hw-genetic-circuits-part-1/index.htmlQ1 What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion DNA Polymerase which synthesises new DNA strands by adding nucleotides using the template during replication. It ensures high precision copying, editing and proofreading. Deoxynucleotides - dNTP consists of a deoxyribose sugar, a nitrogenous base (A, T, C, or G), and three phosphate groups. The DNA polymerase adds them to a growing DNA chain to form the new complementary strand. Reaction Buffer - creates a suitable chemical environment for the Phusion High-Fidelity DNA Polymerase for DNA synthesis. Regulates a stable pH, provides magnesium ions as a cofactor for catalytic activity, and contains important components to increase specificity, yield and high-fidelity performance. Magnesium Chloride - DNA polymerase which synthesises DNA, requires Mg2+ for DNA synthesis, as it is required for enzyme Catalytic Activity, in the active site to catalyze the formation of phosphodiester bonds between the 3′-OH of a primer and the phosphate group of a nucleotide. DMSO to increase G/C rich targets - additive which boosts denaturation of difficult or GC-rich targets. Q2 What are some factors that determine primer annealing temperature during PCR?Hugoen