https://pages.htgaa.org/2026a/tammy-sisodiya/homework/week-10-hw-imaging-and-measurement-technology/index.htmlHomework: Final Project For your final project: Please identify at least one (ideally many) aspect(s) of your project that you will measure. It could be the mass or sequence of a protein, the presence, absence, or quantity of a biomarker, etc. I will use methods to check how effective a protein’s physical and chemical properties (stability, folding, binding) is of my redesigned receptor-binding domains across multiple key metrics: (1) I will measure binding affinity: the thermodynamic strength of the binding between my redesigned T7 tail fiber and the target P. aeruginosa surface receptors (e.g., OprF, PilA). (2) I will measure structural stability using the Root Mean Square Deviation (RMSD) of the polypeptide chain over time to ensure the redesign hasn’t introduced metastability and failure to adopt their native, functional conformation. (3) I will measure solubility and aggregation propensity - this will measure the likelihood of the protein remains soluble versus forming insoluble inclusion bodies during recombinant expression. (4) To ensure functional efficiency and biosafety/biosecurity compliance, I will measure Codon Adaptation Index (CAI) to ensure the elimination of regulated DNA sequences from restricted, highly pathogenic agents (e.g. Ebola, SARS-CoV-2, Anthrax).Hugoen