<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 06 HW: Genetic Circuits Part I :: 2026a-tanishka-kumar</title><link>https://pages.htgaa.org/2026a/tanishka-kumar/homework/week-6-hw-genetic-circuits-part-i/index.html</link><description>Part one 1: What are some components in the Phusion High-Fidelity PCR Master Mix, and what is their purpose? Phusion DNA Polymerase, Nucleotides, Optimised Phusion HF Reaction Buffer with MgCl2 - All at 2X concentration Generates long templates with high accuracy and speed, unattainable with a single enzyme, as per the Thermo Scientific protocol. It states that the error rate of Phusion DNA Polymerase is determined to be 4.4 × 10-7 in Phusion HF Buffer, which is approximately 50-fold lower than that of Thermus aquaticus DNA polymerase, and 6-fold lower than that of Pyrococcus furiosus DNA polymerase. Attached below are graphs that support these claims by comparing with traditional polymerase. The annealing temperature is at 60 degrees for all. https://documents.thermofisher.com/TFS-Assets/LSG/brochures/phusion-high-fidelity-dna-polymerases-flyer.pdf https://documents.thermofisher.com/TFS-Assets/LSG/manuals/MAN0012771_Phusion_HiFi_PCR_MasterMix_100rxn_UG.pdf</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/tanishka-kumar/homework/week-6-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>