https://pages.htgaa.org/2026a/tanishka-kumar/homework/week-9-homework-cell-free-systems/index.htmlPart One: General and Lecturer-Specific Questions 1. Cell-free protein synthesis gives better control over reaction conditions and allows direct addition of molecules like inhibitors or non-natural amino acids. It is useful for producing toxic proteins and for rapid protein prototyping. A cell-free system contains lysate with ribosomes and enzymes, a DNA/mRNA template, amino acids, and buffer salts. These components work together to produce proteins from genetic instructions. ATP regeneration is important because protein synthesis uses large amounts of energy. A creatine phosphate–creatine kinase system can recycle ADP into ATP and maintain continuous protein production.Hugoen