Labs
Lab writeups:
This lab covered standard pipetting and dilution practices. Being an online student, I did not perform the protocol in person. I did refer to the links below to refresh my learning: Significant Figures crash course Dilution Problems, Chemistry, Molarity & Concentration Examples, Formula & Equations
COmpleted in the Homework part of Week 2 .
Completed in Homework part of Week 3
Week 04 HW: Protein Design Part 1
1: Estrogen Receptor Beta (Human) Selected because of its direct relevance to endometriosis, PCOS, and female reproductive health. PDB ID: 1QKM Information about ESR2 from PDB site Protein Classification and Amino acid frequencies Protein structure quality graphs
Lab not part of remote participation.
Week 07 Lab: Neuromorphic Circuits
While during the class I was not able to attend in real time. I caught up the node’s discussion via the recordings upload on the Victoria Node space. I also tried to use Claude to see what it spits out, a lot of irregularity. I also sought help from peers like Aarushi Mishra and under her direct help I attempted several times to create a competitive switch. After several failed attempts, I decided to move forward from this, as I theoretically understand how neuromorphic circuits and lipofectamine transfection work. Having worked in HEK293 transfection before. From what i understand now, The tool Neuromorphic wizard needs to be updated & calibrated to biological understand in a better way. I will be searching more about this later to see if there are better frameworks as open source to use.
Lab not available for remote
Week 10 HW: Protein Design Part 1
See part of Homework in Week 10 Homework space.
See week 11 Homework.
Post-lab Questions 1.Which genes when transferred into E. coli will induce the production of lycopene and beta-carotene, respectively? crtE, crtB, and crtL produce lycopene. Adding crtY produces beta-carotene. 2.Why do the plasmids that are transferred into the E. coli need to contain an antibiotic resistance gene? Antibiotic resistance keeps only plasmid-containing cells alive, ensuring plasmid maintenance.