I am curious about exploring the potential of HTGAA utilising the Design Build Test and Learn strategy. I have recently done an Innovate UK project in which I used MoClo kits to design the synthesis of xanthone in yeast. I am curious about exploring the potential of the phenylpropanoid pathway and the plethora of useful chemicals that could be produced.
Part 1: Benchling & In-silico Gel Art Simulate Restriction Enzyme Digestion
Part 3: DNA Design Challenge 3.1. Choose your protein Retrieved Germin like protein sequence located on chromosome 8, important in plants biotic and abiotic stresses.
You can view/download my presentation here: Week 3 Homework PPT
Subsections of Homework
Week 1 HW: Principles and Practices
I am curious about exploring the potential of HTGAA utilising the Design Build Test and Learn strategy. I have recently done an Innovate UK project in which I used MoClo kits to design the synthesis of xanthone in yeast. I am curious about exploring the potential of the phenylpropanoid pathway and the plethora of useful chemicals that could be produced.
Hence, I am proposing this study “Engineered Naringenin production through Design Build Test and Learn Strategy”, as a committed listener, for which I would be interested to work in Victoria,Genspace or Designer Cells.I am currently based in UK and hence intereted in working on nodes in UK.
The study involving the metabolic engineering of Naringenin will be conducted under approved BSL-1 laboratory conditions, in accordance with institutional and biosafety guidelines. For in vitro cell cultures, commercially available human-derived cell lines will be used. No primary human subjects will be directly involved. All data collection, analysis and reporting will adhere to the principles of ethical research conduct.
Purpose
Naringenin (C15H12O5) is a novel bioactive compound within the flavonoid group, found naturally in the skin of citrus fruits. This citrus flavonoid is well known for its anti-inflammatory, antioxidant, and anticarcinogenic properties, and can support skin against the detrimental impacts of inflammaging. Outside skin care research, Naringenin has shown promising results in treating issues like obesity and cardiovascular diseases.
The primary source of Naringenin is citrus fruits, hence consuming a lot of natural resources and labour. What if we make the process economical and produce naringenin at lab scale? Sounds great and doable through synthetic biology.
Design
I propose engineering the phenylpropanoid pathway in S. cerevisiae to produce naringenin, utilizing the Design Build Test and Learn (DBTL) strategy of MoClo kits.
Work Packages
WP1: Engineering the key enzymes in the phenylpropanoid to flavonoid pathway for naringenin biosynthesis
WP2: Confirming the expression through proteomic and metabolomic analysis
WP3: Formulation development for enhanced solubility and bioavailability
WP4: Biological evaluation on skin models
Assumptions
The proposed study holds the potential to revolutionise the cosmetic industry by providing an economical source of Naringenin without relying on natural resources, hence a step towards preventing the depletion of natural resources.
Risks of Failure & Success
The study requires step-by-step, careful pathway optimisation through omics approaches to identify any bottlenecks in the engineering strategy in a heterologous host. If not followed accurately, the pathway will not lead to Naringenin production.
With attention to detail and proper record keeping, the project holds the potential to revolutionize the cosmetic industry.
References
Cai, J., Wen, H., Zhou, H., Zhang, D., Lan, D., Liu, S., & Zhang, J. (2023). Naringenin: A flavanone with anti-inflammatory and anti-infective properties. Biomedicine & Pharmacotherapy, 164, 114990.
Manchope, M. F., Casagrande, R., & Verri Jr, W. A. (2016). Naringenin: an analgesic and anti-inflammatory citrus flavanone. Oncotarget, 8(3), 3766.
Österlund, C., Hrapovic, N., Lafon-Kolb, V., Amini, N., Smiljanic, S., & Visdal-Johnsen, L. (2023). Protective effects of naringenin against UVB irradiation and air pollution-induced skin aging and pigmentation. Cosmetics, 10(3), 88.
Rashmi, R., Magesh, S. B., Ramkumar, K. M., Suryanarayanan, S., & SubbaRao, M. V. (2018). Antioxidant potential of naringenin helps to protect liver tissue from streptozotocin-induced damage. Reports of Biochemistry & Molecular Biology, 7(1), 76.
Biosecurity & Safety Considerations
Consideration
Option 1
Option 2
Option 3
Enhance Biosecurity
By preventing incidents
✓
By helping respond
✓
Foster Lab Safety
By preventing incidents
✓
By helping respond
✓
Protect the Environment
By preventing incidents
✓
By helping respond
Other Considerations
Minimizing costs and burdens to stakeholders
✓
Feasibility
✓
Not impede research
✓
Promote constructive applications
✓
I will propose constructive applications of the proposed research in the healthcare and beauty sectors, and not impede research and researchers. The governance action to be addressed should focus on the rights of the worker/researcher. As many projects are led by industry, researchers must not be treated as paid labour but are provided fair recognition, intellectual property rights, and equal participation in patent ownership.
I am extremely thankful to be a part of HTGAA-2026, looking forward to learning from world leading researchers in the field of synthetic biology.
Kind Regards, Tehseen
Homework 2 - DNA READ,WRITE,& EDIT
Part 1: Benchling & In-silico Gel Art
Simulate Restriction Enzyme Digestion
Part 3: DNA Design Challenge
3.1. Choose your protein
Retrieved Germin like protein sequence located on chromosome 8, important in plants biotic and abiotic stresses.
Done using IDT tool as Twist Bioscience link provided was not working, optimised for expression in S. cerevisiae, removed type II restriction enzymes BsaI, BsmBI and BpsI.
The sequence entered of OsRGLP1 having start codon and a stop codon:
ATG GCT TCT GAT CCA TCT CCA TTA CAA GAT TTC TGT GTT GCT GAC ATG CAT TCT
CCA GTT TTG GTC AAC GGT TTT GCT TGT TTG AAC CCA AAG GAT GTC AAT GCT GAT
CAT TTC TTC AAG GCT GCT ATG TTG GAC ACT CCA AGA AAG ACC AAC AAG GTT GGT
TCC AAT GTC ACT TTG ATC AAC GTT ATG CAA ATT CCA GGT TTG AAC ACT TTG GGT
ATC TCC ATT GCC AGA ATT GAT TAT GCT CCA TTG GGT CAA AAC CCA CCA CAC ACA
CAC CCA AGA GCC ACT GAA ATT TTG ACT GTT TTG GAA GGT ACT TTG TAT GTT GGT
TTT GTC ACT TCC AAC CCA GAC AAC AAG TTC TTC TCC AAG GTT TTG AAC AAG GGT
GAT GTC TTT GTC TTC CCA GTT GGT TTG ATT CAC TTC CAA TTC AAC CCA AAC CCA
TAC AAG CCA GCT GTT GCT ATT GCT GCT TTA TCT TCT CAA AAC CCA GGT GCC ATC
ACC ATT GCC AAT GCT GTT TTT GGT TCC AAG CCA CCA ATT TCT GAT GAT GTT TTG
GCC AAG GCT TTC CAA GTT GAA AAG GGT ACC ATT GAT TGG TTA CAA GCT CAA TTC
TGG GAA AAC AAC CAC TAC TAA
These are the parameters used on the IDT codon optimisation tool; restriction sites to avoid were entered. However, when checking the sequence on Benchling for the absence of these enzymes, BsmBI and BsaI are not present, but one restriction site of BbsI does exist. The codon optimisation might have been more accurate if Twist or GenWiz software had been used.
3.4. You have a sequence! Now what?
The gene sequence can be recombinantly expressed in S. cerevisiae by using a protocol adapted from Gietz, R. D et al., 2007.
Part 4: Prepare a Twist DNA Synthesis Order
Build Your DNA Insert Sequence
Added 5’ and 3’ sequences for Type II restriction digestion and have annotated the sequence 5’ end - CDS - 3’ end. Can be subsequently used for ordering through Twist Bioscience.
Used vectors of YTK kit and used the combinatorial assembly feature of Benchling to design the final vector: pYtk009 vector (pTDH3) promoter, pYTK051 vector (tENO1) terminator.
