https://pages.htgaa.org/2026a/tianyi-terry-luo/homework/week-06-hw-genetic-circuits-part-i/index.htmlPart A: DNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity PCR Master Mix is a 2X mix containing Phusion DNA Polymerase, nucleotides, and an optimized reaction buffer including MgCl₂. Functionally, the polymerase synthesizes the new DNA strand, the dNTPs are the nucleotide building blocks it incorporates, the buffer maintains the reaction chemistry, and Mg²⁺ is the essential cofactor that enables polymerase activity and helps stabilize primer-template interactions. Phusion is called “high fidelity” because it has 3′→5′ exonuclease proofreading activity, which lowers the error rate compared with standard Taq-type PCR enzymes. What are some factors that determine primer annealing temperature during PCR?Hugoen