<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 10 HW: Imaging and Measurement :: 2026a-tianyi-terry-luo</title><link>https://pages.htgaa.org/2026a/tianyi-terry-luo/homework/week-10-hw-imaging-and-measurement/index.html</link><description>Homework: Final Project For the final project, I will primarily measure whether my designed DNA templates are correct and whether they can produce proteins in a T7 cell-free expression system. I will verify the DNA constructs using DNA sequencing and agarose gel electrophoresis, then test expression using two templates: a minimal ureABC structural urease construct and a separate sfGFP reporter construct. The sfGFP template will serve as a visual positive control, and its expression will be measured by fluorescence, while expression of UreA, UreB, and UreC will be measured using SDS-PAGE. I may also perform an exploratory urease-related activity assay, but this is not the main measurement because the minimal structural construct does not include the full accessory genes required for strong urease activation. Homework: Waters Part I — Molecular Weight Based on the predicted amino acid sequence of eGFP (see below) and any known modifications, what is the calculated molecular weight?</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/tianyi-terry-luo/homework/week-10-hw-imaging-and-measurement/index.xml" rel="self" type="application/rss+xml"/></channel></rss>