<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part I :: 2026a-tuzun-guvener</title><link>https://pages.htgaa.org/2026a/tuzun-guvener/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Assignment: DNA Assembly Answer the following questions about the protocol in:
1. What are some components in the Phusion High-Fidelity PCR Master Mix, and what is their purpose?
Below are the components found in the New England Biolab’s Phusion HF PCR Master Mix:
•Phusion DNA Polymerase: It performs 5’ → 3’ polymerase activity and 5’ → 3’exonuclease (proofreading) activity with greater fidelity, &gt;50x better in comparison to regular Taq polymerase. Because it is a fusion polymerase with an Sso7d domain, it adds nucleotides more quickly, reducing the required extension time. Due to the exonuclease activity. Phusion polymerase produces blunt ends.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/tuzun-guvener/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>