Homework

Weekly homework submissions:

• Week 1 HW: Principles and Practices

  At the core of the project is the development of an improved bioprinter designed for two-color bacterial printing using two strains of Escherichia coli: a non-pathogenic, non-modified strain and a genetically transformed strain carrying a plasmid encoding the expression of a color marker. This approach enables the creation of visually distinguishable bacterial images and expands both the artistic and research potential of bioprinting.

• Week 2 HW: DNA read, write and edit

  Part 1: Benchling & In-silico Gel Art

• Week 5 HW: Protein design part II

  Part A: SOD1 Binder Peptide Design (From Pranam) Part 1: Generate Binders with PepMLM the human SOD1 sequence (P00441): MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ the A4V mutant SOD1 sequence: MATKVVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ four peptides of length 12 amino acids conditioned on the mutant SOD1 sequence with the known SOD1-binding peptide FLYRWLPSRRGG for comparison: