https://pages.htgaa.org/2026a/vithushan-varatharaj/homework/week-09-hw-cell-free-systems/index.htmlGeneral Homework Questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. One advantage includes the fact that cell-free protein synthesis gives direct control over reaction composition which means we can precisely control the exact concenctrations of factors like DNA, amino acids and modified nucleotides. No cell membrane barrier means everything is immediately accessible and modifiable The speed at which you can cycle designs and tests is much faster thant that if you’d have to do the usual process of cloning then transform then grow You can precisely and exactly control the specific environments its in, setting the exact pH, temperature and also be able to eliminate toxic constraints. The bread and butter for cell free systems is the cell extract which includes the ribosomes that are the machinery for protein synthesis, tRNAs to deliver amino acids, translation factors and also the enzymes required for and involved in metabolism.Hugoen