https://pages.htgaa.org/2026a/vivien-roussel/homework/week-06-hw-gentetic_circuit_i/index.htmlTable of Contents Assignment: DNA Assembly 1. Components of the Phusion High-Fidelity PCR Master Mix 2. Factors determining primer annealing temperature 3. PCR vs restriction enzyme digests 4. Ensuring compatibility for Gibson cloning 5. Plasmid DNA transformation into E. coli 6. Golden Gate Assembly Benchling / Modeling Component Asimov Kernel Assignment — Repository and Circuit DesignHugoen