Week 1 HW: Principles and Practices

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  1. Describe a biological engineering application or tool you want to develop and why. One application that might be worth exploring would be in the realm of data storage for an imagined, hypothetical semi-exposed media. For this context special proteins could be designed in applications that bind to sequences that code for hidden malware or faulty sequences and or act for preserving media. I imagine that both this media design and this protein use would be niche but nonetheless fun to design for. Modalities could be as a gentle spray or as a settled solution that could be extracted.

  2. Describe one or more governance/policy goals related to ensuring that this application or tool contributes to an “ethical” future, like ensuring non-malfeasance (preventing harm).

A policy goal to ensure that this application contributes to an ethical future could be ensuring transparent design and standardization of these proteins (and their stats per context) so there is a way to validate their production and application prior to use. This could reduce the chance of bad actors sabotaging batches or distributing lower quality batches.

  1. Describe at least three different potential governance “actions” by considering the four aspects below (Purpose, Design, Assumptions, Risks of Failure & “Success”)
Governance ActionPurposeDesignAssumptionsFailure and Success Risks
Standardized LibrariesEducates communities on proteins available and safe designsCreate a library to hold these designs, as well as protocols for their manufacture to specThat communities will agree on these standards and that there are not unnecessary inequities holding these standardsSuccess means that manufacture paths are straightforward.

Failure means that design paths are too numerous to account for and fragmentation possibilities are higher
Blue-teaming DesignTo build a community line of protection of designs and applicationsDesign blue teaming frameworks and recruit educators to practiceThat there is sufficient interest for fundingSuccess means that it is easier for production and share. Failure means that this pipeline has an established line of people who
Red-teaming DesignTo identify means of sabotaging to proteins to protect libraries and distributorsDesign blue-teaming framework and recruit educators to practiceThat there is sufficient interest for fundingSuccess means that it is easier for production and share. Failure means that this pipeline has less visibility on sabotage routes
  1. Next, score (from 1-3 with, 1 as the best, or n/a) each of your governance actions against your rubric of policy goals.
Does the option:Option 1Option 2Option 3
Enhance Biosecurity333
• By preventing incidents333
• By helping respond333
Foster Lab Safety333
• By preventing incident333
• By helping respond333
Protect the environment333
• By preventing incidents333
• By helping respond333
Other considerations333
• Minimizing costs and burdens to stakeholders322
• Feasibility?333
• Not impede research333
• Promote constructive applications333
  1. Last, drawing upon this scoring, describe which governance option, or combination of options, you would prioritize, and why. Outline any trade-offs you considered as well as assumptions and uncertainties.

I would prioritize library standardization. All operations, from that educators to users, can be facilitated from having a common reference frame. Further, among the options, this appears the most neutral. Overall, proper security requires practitioners from both sides of the spectrum: training in protecting and disrupting systems, and being willing to document for the good of the community.


Homework

Answers for Homework Questions from Professor Jabson:

  1. Error rate - 1: 10^6. This is dwarfed by the length of the human genome. The body has numerous selectivity and repair mechanisms to deal with mitigate issues from mutations.
  2. 64 / Some codes might not work due to chemical incompatibility and structural reasons.

Answers for Homework Questions from Dr. LeProust:

  1. Phosphoramidite DNA Synthesis Cycle
  2. Increasing inefficiency of reactions with greater length
  3. Extremely high inefficiency Error rates

Answers for Homework Question from George Church:

  1. Arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine

I’m still forming my thoughts on how this affects my view of the Lysine Contingency.