https://pages.htgaa.org/2026a/yajaira-kelita-mellado-villanueva/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. 2. Describe the main components of a cell-free expression system and explain the role of each component. 3. Why is energy provision regeneration critical in cell-free systems? Describe a method you could use to ensure continuous ATP supply in your cell-free experiment. 4. Compare prokaryotic versus eukaryotic cell-free expression systems. Choose a protein to produce in each system and explain why. 5. How would you design a cell-free experiment to optimize the expression of a membrane protein? Discuss the challenges and how you would address them in your setup. 6. Imagine you observe a low yield of your target protein in a cell-free system. Describe three possible reasons for this and suggest a troubleshooting strategy for each. Homework question from Kate Adamala Design an example of a useful synthetic minimal cell as follows:Hugoen