https://pages.htgaa.org/2026a/yanchen-shen/homework/week-11-hw-building-genomes/index.htmlA. Master Mix Component Analysis E. coli Lysate BL21 (DE3) Star Lysate: Provides the cellular machinery for transcription and translation, including ribosomes and T7 RNA polymerase. The “Star” mutation helps reduce mRNA degradation. Salts / Buffer Potassium Glutamate: Maintains ionic strength and supports ribosome activity. HEPES-KOH pH 7.5: Keeps the reaction at a stable pH. Magnesium Glutamate: Supplies magnesium ions needed for ribosomes and enzymes. Potassium Phosphate: Acts as a buffer and supports ATP regeneration.Hugoen