https://pages.htgaa.org/2026a/yao-wang/homework/week-06-hw-genetic-circuits-part-i-assemblytechnologies/index.htmlAnswers to Questions About This Week’s Lab Protocol 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity PCR Master Mix typically contains several important components: Phusion DNA polymerase, dNTPs, reaction buffer, and MgCl2. The polymerase synthesizes new DNA strands and has proofreading activity, which lowers the error rate compared with standard Taq polymerase. The dNTPs provide the nucleotide building blocks needed to make the new DNA strands. The buffer maintains the proper chemical environment, including pH and salt concentration, for the enzyme to work efficiently. MgCl2 is an essential cofactor that allows the polymerase to function properly. In this lab, the master mix is provided as a 2X mix, so only template DNA, primers, and water are added separately. (neb.com)Hugoen