https://pages.htgaa.org/2026a/yutong-wu/homework/week-10-hw-imaging-and-measurement/index.htmlWhat to measure: Identity, mass, purity, and post-translational modifications of the target protein; concentration of a biomarker; oligomeric state. How: Intact mass by LC-MS (QTof) → confirms overall MW and detects unexpected modifications. Peptide mapping by tryptic digest + LC-MS/MS → confirms primary sequence and identifies PTM sites. Native MS / CDMS → reveals folded state and oligomeric assembly. SDS-PAGE / Western blot → quick purity and identity check before MS. UV-Vis (A280) → concentration. Part I — Molecular Weight of Intact eGFP Q1. Theoretical MW from sequence Sequence length: 247 residues (includes LE linker + HHHHHH His-tag).Hugoen