https://pages.htgaa.org/2026a/yutong-wu/homework/week-11-hw-bioproduction/index.htmlUnfortunately I was away at CHI 2026 during the contribution window, so I didn’t get to commit a pixel in time. Part B – Cell-Free Protein Synthesis B1. Role of each component E. coli Lysate BL21 (DE3) Star Lysate (with T7 RNAP): The “factory floor” – a crude cytoplasmic extract carrying ribosomes, tRNAs, aminoacyl-tRNA synthetases, translation factors, and the T7 RNA polymerase needed to transcribe T7-promoter templates. The DE3 Star background also lacks RNase E activity, so mRNAs last longer. Salts / BufferHugoen