<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part 1 :: 2026a-yuxin-wang</title><link>https://pages.htgaa.org/2026a/yuxin-wang/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Assignment: DNA Assembly 1.What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose?
Phusion is an enzyme commonly used for high-precision cloning. Its Master Mix typically contains the following core components:Phusion DNA Polymerase: The core enzyme. It possesses 3’→5’ exonuclease activity (proofreading function), ensuring high fidelity DNA replication with an extremely low error rate. dNTPs (dATP, dTTP, dCTP, dGTP): The “raw materials” for building new DNA strands.Mg²⁺ (usually MgCl₂): A cofactor for the polymerase. It stabilizes primer-template binding and activates the enzyme’s catalytic center. Buffer: Maintains a constant pH and provides suitable ionic strength to ensure enzyme activity.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/yuxin-wang/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>