https://pages.htgaa.org/2026a/yuxin-wang/homework/week-11-hw-building-genomes/index.htmlPart B: Cell-Free Protein Synthesis | Cell-Free Reagents Recording to Gemini： Component Roles in CFPS E. coli Lysate (BL21 (DE3) Star): Provides the essential molecular machinery, including ribosomes and chaperones, while the T7 RNA Polymerase drives the transcription of DNA into mRNA. HEPES-KOH pH 7.5: Functions as a chemical buffer to maintain a stable pH, ensuring that enzymes and proteins remain folded and active throughout the reaction.Hugoen