<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part I :: 2026a-zaira-guzman</title><link>https://pages.htgaa.org/2026a/zaira-guzman/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Part A: Assignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Based on standard molecular biology principles, a High-Fidelity PCR Master Mix typically contains
Phusion DNA Polymerase: A highly processive, proofreading polymerase responsible for synthesizing the new DNA strands accurately. Deoxynucleotide Triphosphates (dNTPs): The A, T, C, and G building blocks that the polymerase uses to build the new DNA strand. Reaction Buffer: Maintains the optimal pH and salt conditions for the enzyme. Magnesium Chloride: A crucial cofactor required for DNA polymerase activity. Specific template DNA, primers, and nuclease-free water. 2. What are some factors that determine primer annealing temperature during PCR?</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/zaira-guzman/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>