https://pages.htgaa.org/2026a/zaira-guzman/homework/week-09-hw-cell-free-systems/index.htmlHomework Part A: General and Lecturer-Specific Questions General homework questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free systems provide an open-access biological engine where you have direct control over every molecular dial. The main advantage over traditional in vivo methods is the lack of a cellular wall, which allows for the direct addition of non-canonical amino acids or specific inhibitors. This transparency makes it perfect for producing proteins that are normally toxic to a living host or for rapid prototyping where results are needed in hours rather than days. Two key cases where this is superior include the production of antimicrobial peptides that would lyses a host cell and the synthesis of proteins containing site-specific labels for structural NMR analysisHugoen