https://pages.htgaa.org/2026a/zeynep-begum-kara/homework/week-06-genetic-circuits-i/index.html1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion HF PCR Master Mix is a ready-to-use 2X solution that contains everything needed for PCR except the template, primers, and water. The most important component is Phusion DNA Polymerase, which is the enzyme that copies DNA during the reaction. The standard alternative is Taq polymerase, a widely used enzyme isolated from a heat-resistant bacterium called Thermus aquaticus. Taq survives the high temperatures of PCR but has no proofreading ability, so it cannot correct mistakes it makes while copying, resulting in a relatively high error rate. Phusion addresses this by including a proofreading domain that catches and fixes errors as they occur, making it about 50 times more accurate than Taq. This accuracy is important in a mutagenesis experiment where only the specific, intended mutations should be introduced and any unintended errors would compromise the result.Hugoen