LOCUS       Reflectin_A1_Expression_Cassette in pTwist Amp High Copy                3481 bp DNA     circular   18-FEB-2026
DEFINITION                               Reflectin_A1_Expression_Cassette in pTwist Amp High Copy
COMMENT                                  Resistance markers: Ampicillin

COMMENT                                  This file was created using tools provided by Twist Bioscience
FEATURES                                 Location/Qualifiers
     source                              1..3481
                                         /organism="Reflectin_A1_Expression_Cassette in pTwist Amp High Copy"
     misc_feature_                       1..1260
                                         /label="insert"
     misc_feature_                       1106..1122
                                         /label="6xHis"
     misc_feature_                       1143..1186
                                         /label="rrnB_T1"
     misc_feature_                       1227..1246
                                         /label="stem_loop_early_T7"
     misc_feature_                       1261..3481
                                         /label="vector_backbone-1260-3481"
     misc_feature_                       1317..1333
                                         /label="m13_reverse"
     misc_feature_                       1505..2093
                                         /label="colE1_high_copy"
     misc_feature_                       2210..2234
                                         /label="attB1"
     misc_feature_                       2334..3194
                                         /label="ampR"
     misc_feature_                       3195..3299
                                         /label="AmpR_promoter"
ORIGIN
        1 TTTACGGCTA GCTCAGTCCT AGGTATAGTG CTAGCCATTA AAGAGGAGAA AGGTACCATG
       61 AATCGCTATC TGAATCGGCA ACGTCTGTAT AATATGTATC GTAATAAATA CCGCGGTGTT
      121 ATGGAGCCCA TGAGTCGTAT GACTATGGAT TTCCAGGGTC GCTATATGGA TTCTCAGGGG
      181 CGCATGGTCG ATCCGCGCTA TTATGATTAT TACGGGCGTA TGCATGACCA TGACAGATAT
      241 TATGGAAGAT CGATGTTTAA CCAGGGTCAT TCAATGGACA GCCAACGCTA TGGTGGTTGG
      301 ATGGACAATC CGGAGCGCTA TATGGATATG TCTGGTTATC AGATGGATAT GCAGGGTCGT
      361 TGGATGGACG CCCAGGGACG GTTCAATAAC CCATTCGGAC AGATGTGGCA TGGTCGTCAG
      421 GGCCATTACC CGGGTTATAT GTCCAGCCAC TCAATGTATG GTAGAAACAT GTACAACCCG
      481 TATCATAGTC ATTATGCTAG CCGCCATTTT GATAGCCCTG AACGGTGGAT GGATATGAGT
      541 GGGTACCAAA TGGATATGCA AGGTAGATGG ATGGATAATT ATGGACGGTA CGTAAATCCT
      601 TTTAACCACC ACATGTACGG TCGGAATATG TGCTATCCGT ACGGCAACCA CTATTACAAC
      661 CGGCACATGG AACATCCGGA ACGTTATATG GATATGTCTG GTTACCAAAT GGATATGCAA
      721 GGGCGCTGGA TGGATACACA TGGTCGCCAT TGTAATCCGT TTGGCCAGAT GTGGCATAAC
      781 AGACACGGGT ACTATCCGGG TCATCCGCAT GGCCGAAATA TGTTTCAGCC AGAACGTTGG
      841 ATGGATATGA GTGGCTATCA AATGGATATG CAGGGGCGGT GGATGGACAA TTACGGCAGA
      901 TACGTTAACC CATTCAGCCA TAATTATGGT CGCCATATGA ATTACCCGGG GGGTCATTAT
      961 AACTATCACC ATGGCCGGTA TATGAACCAC CCCGAAAGAC ACATGGACAT GTCCTCATAT
     1021 CAGATGGATA TGCATGGCAG ATGGATGGAT AATCAAGGAC GTTACATTGA TAATTTTGAT
     1081 CGTAACTATT ATGACTATCA CATGTATCAT CACCATCACC ATCATCACTA ACCAGGCATC
     1141 AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG
     1201 TGAACGCTCT CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA
     1261 AGGCTAGGTG GAGGCTCAGT GATGATAAGT CTGCGATGGT GGATGCATGT GTCATGGTCA
     1321 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCAGAGGGC ACAATCCTAT TCCGCGCTAT
     1381 CCGACAATCT CCAAGACATT AGGTGGAGTT CAGTTCGGCG TATGGCATAT GTCGCTGGAA
     1441 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     1501 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     1561 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     1621 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     1681 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     1741 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     1801 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     1861 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     1921 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     1981 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     2041 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
     2101 CTTTGATCTT TTCTACGGGG TCTGACGCTC TATTCAACAA AGCCGCCGTC CCGTCAAGTC
     2161 AGCGTAAATG GGTAGGGGGC TTCAAATCGT CCTCGTGATA CCAATTCGGA GCCTGCTTTT
     2221 TTGTACAAAC TTGTTGATAA TGGCAATTCA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2281 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2341 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2401 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2461 GCAATGATAC CGCGAGAGCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2521 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2581 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2641 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2701 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2761 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2821 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2881 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2941 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     3001 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     3061 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     3121 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3181 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3241 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3301 ACATTTCCCC GAAAAGTGCC AGATACCTGA AACAAAACCC ATCGTACGGC CAAGGAAGTC
     3361 TCCAATAACT GTGATCCACC ACAAGCGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
     3421 ACGGCCAGTC ATGCATAATC CGCACGCATC TGGAATAAGG AAGTGCCATT CCGCCTGACC
     3481 T
//