Week 6 HW: Genetic Circuits Part I

ASIMOV KERNEL REPRESSILATOR

Original repressilator construct from the example repo, with its simulation data:

My simulation graphs did not match: Even after replacing all the parts specifically for the ones in the Characterized Bacteria repo, it still didn’t match. I will have to debug further.

I asked AI to help me debug and it said that some possible reasons might be that the parts I am using might have different metadata even if they appear identical, that my simulation window might be too long, that the RBS I chose might be too weak or strong, that the backbone or copy-number might differ (I tried with and without an added backbone), that the promoters might not have the right repressor behavior… many alternatives to test!