https://pages.htgaa.org/2026b/ahmed-hussain-shah/week-02-hw-dna-design/index.htmlPart 0: Kernel Setup My work is located in the HTBAA 2026 workspace, in the repository ahmed-synbio-ink under the folder shah-hw2. Part 1: Define your expression target Molecule: LbCas12a (CRISPR-Cas effector nuclease) engineered as a fusion protein with an Elastin-Like Polypeptide (ELP) tag and a mutated Mxe GyrA Intein. This is the core diagnostic engine for the “SynBio-Ink” multiplexed biosensor. Sequence: Lachnospiraceae bacterium Cas12a (UniProt Accession: A0A182DWE3). Host/Chassis: E. coli Cell-Free Protein Synthesis (CFPS) extract. Destination: The protein is expressed directly into the open in vitro diagnostic matrix (the “ink”). Therefore, no signal peptides or cellular secretion tags are required. Part 2: Design your genetic parts To eliminate the massive cost of downstream affinity chromatography for the bio-ink, I designed a self-cleaving thermal purification cassette.Hugoen