LOCUS       AW3T7_PETase            2696 bp    DNA     circular SYN 07-OCT-2026
DEFINITION  Aimer_W3_T7_PETase: exact official Ginkgo CFPS template m9307404.
ACCESSION   AW3T7_PETase
VERSION     AW3T7_PETase
KEYWORDS    T7; cell-free protein synthesis; HTBAA Week 3.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            other sequences; artificial sequences.
COMMENT     Prepared locally for Aimer Koshmambetov, Designer Cells at Yonsei
            University. No Kernel construct creation is implied.
COMMENT     DNA is copied from the official linked Coopetition specification.
COMMENT     Tagged protein translation exactly matches official Week 3 assignment.
COMMENT     Full DNA sequence has not been modified or codon optimized.
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="Official source:
                     https://docs.google.com/document/d/1E9RKXezEU5pMO5A10kkUHjHrfL5f8segL0QeNHXAs_E"
                     /note="Ginkgo template m9307404; circular topology
                     declared in official specification"
     CDS             complement(17..832)
                     /label="KanR_official_source_variant"
                     /product="kanamycin resistance protein"
                     /codon_start=1
                     /transl_table=1
                     /note="Complete source ORF; 814/816 nt match to Tn903
                     V00359.1 CDS 1162..1977. Official sequence preserved,
                     including Arg12Lys and synonymous Pro184Pro variants."
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    938..1557
                     /label="pUC19_homologous_backbone_region"
                     /note="Exact 620/620 nt match to M77789.2 positions
                     1900..2519. pJL1 replication backbone supported by primary
                     sources. Full replication origin boundaries are not
                     independently established."
     regulatory      1573..1589
                     /regulatory_class="promoter"
                     /label="T7_promoter_recognition_motif"
                     /note="Exact 17 nt motif in unchanged official DNA;
                     interval marks recognition motif, not independently
                     verified full promoter boundary."
     regulatory      1623..1645
                     /regulatory_class="ribosome_binding_site"
                     /label="T7_gene10_extended_RBS_motif"
                     /note="Exact 23 nt motif in unchanged official DNA;
                     interval does not establish the full 5-prime UTR
                     boundary."
     regulatory      2648..2696
                     /regulatory_class="terminator"
                     /label="T7_terminator_motif"
                     /note="Exact 49 nt motif in unchanged official DNA;
                     interval marks the motif, not an experimentally
                     established functional boundary."
     CDS             1653..2582
                     /label="PETase_official_tagged_CDS"
                     /product="PETase (exact official tagged protein sequence)"
                     /codon_start=1
                     /transl_table=1
                     /note="Translation verified exactly against assignment:
                     https://docs.google.com/document/d/1ek2LEZNJg_uxMsX2MXm4123-0NTT-65CAwqqr0-YiXA"
                     /translation="MEKKIWSHPQFEKGGSGENLYFQSGGASPAGGGANPYERGPNPT
                     DALLEASSGPFSVSEENVSRLSASGFGGGTIYYPRENNTYGAVAISPGYTGTEASIAW
                     LGERIASHGFVVITIDTITTLDQPDSRAEQLNAALNHMINRASSTVRSRIDSSRLAVM
                     GHSMGGGGTLRLASQRPDLKAAIPLTPWHLNKNWSSVTVPTLIIGADLDTIAPVATHA
                     KPFYNSLPSSISKAYLELDGATHFAPNIPNKIIGKYSVAWLKRFVDNDTRYTQFLCPG
                     PRDGLFGEVEEYRSTCPFGGSGVSGWRLFKKIS"
ORIGIN
        1 tgaaagccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
       61 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      121 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
      181 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
      241 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
      301 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
      361 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
      421 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
      481 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
      541 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
      601 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
      661 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
      721 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
      781 tggaatttaa tcgcggcttc gagcaagacg tttcccgttg aatatggctc ataacacccc
      841 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
      901 gtgcaatgta acatcagaga ttttgagaca caacgtgaga tcaaaggatc ttcttgagat
      961 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1021 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1081 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcgatc
     1561 ccgcgaaagt cttaatacga ctcactatag ggagaccaca acggtttccc tctagaaata
     1621 attttgttta actttaagaa ggagatatac atatggagaa gaagatatgg tcgcatccac
     1681 aattcgagaa gggcggctca ggagaaaact tatactttca aagtggcggc gcgagcccgg
     1741 caggcggcgg tgccaacccc tacgagcgtg gcccaaaccc caccgacgcg ttgctcgaag
     1801 ccagttcagg tcctttctct gtctctgagg agaacgtctc aagattgtca gctagcggtt
     1861 ttggtggtgg gaccatatac tacccccgcg agaacaatac ttacggcgcg gtggccatat
     1921 ctccaggcta cacaggtaca gaagcctcga ttgcttggct gggtgaacgc attgccagcc
     1981 acggattcgt agtcatcacg atcgatacca taacaacgct ggaccagccc gactcacgcg
     2041 ctgagcaatt gaatgccgca cttaatcaca tgatcaaccg agcgtcttcc acggttcgct
     2101 ctcggatcga ctcctctcgc ttagcagtga tgggacatag catgggtggc ggcggcaccc
     2161 tgagactggc aagccagcgc ccggacctga aagccgctat accgctcacg ccttggcact
     2221 taaacaagaa ctggtcttcc gtgacggttc ccacattgat aataggagca gatttagaca
     2281 ccatcgcccc ggtagccacg cacgctaagc ctttctacaa tagcctgccg tctagtattt
     2341 caaaggccta ccttgagctc gacggcgcca cgcactttgc tcctaatatc ccgaacaaaa
     2401 tcattggcaa atactctgtc gcgtggttaa agcgcttcgt agacaatgat acccgataca
     2461 cccaatttct gtgccccggc ccgcgtgacg gtttattcgg agaagtcgaa gaataccgat
     2521 ccacgtgccc gttcggtggt tctggtgtga gcggctggcg gctgttcaag aagattagct
     2581 aagtcgaccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     2641 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgc
//